TCR Vα chain expression influences reactivity to the hapten TNP
نویسندگان
چکیده
We have recently demonstrated a remarkable selection of in vitro cultivated, TNP-specific polyclonal T cell lines for the expression of a TCR β chain encoded by the Vβ8.2 gene. The goal of the present study was to analyse Vα usage in Vβ8.2 T cells responsive to TNP, using TNP-specific T cell lines derived from three common strains of mice, as well as from Vβ8.2 transgenic mice. Results indicate that in vitro TNP stimulation of T cells from TNP-immune mice results in significant skewing of Vα usage among responding Vβ8.2 T cells, with overexpression observed for Vα3.2 and Vα8. These results indicate that Vα expression influences recognition of TNP by T cells, and suggest that the hapten TNP might be recognized like typical peptide antigens by combinatorial TCR α and β contact sites.
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